CD86 Protein (CD86) (AA 26-247) (Fc Tag)
-
- Target Alle CD86 Proteine anzeigen
- CD86
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 26-247
-
Spezies
- Human
-
Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses CD86 Protein ist gelabelt mit Fc Tag.
- Verwendungszweck
- Human B7-2/CD86 Protein
- Sequenz
- Leu26-Pro247
- Produktmerkmale
- Recombinant Human B7-2/CD86 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu26-Pro247.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CTLA-4, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human B7-2, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
-
-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- CD86
- Andere Bezeichnung
- B7-2 (CD86 Produkte)
- Synonyme
- B7-2 Protein, B7.2 Protein, B70 Protein, CD28LG2 Protein, LAB72 Protein, B7 Protein, CLS1 Protein, Cd28l2 Protein, ETC-1 Protein, Ly-58 Protein, Ly58 Protein, MB7 Protein, MB7-2 Protein, TS/A-2 Protein, CD86 molecule Protein, CD86 antigen Protein, integrin subunit alpha 2b Protein, CD86 Protein, Cd86 Protein, Itga2b Protein
- Hintergrund
- B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
- Molekulargewicht
- 52 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
- Pathways
- T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
-