AXL Protein (AA 33-449) (His tag)

Produktnummer ABIN7273880

Produktdetails

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Protein-Typ: Recombinant

Proteineigenschaft: AA 33-449

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses AXL Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus AXL Protein

Sequenz: Glu33-Pro449

Produktmerkmale: Recombinant Cynomolgus AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Pro449.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus AXL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Andere Bezeichnung: AXL (AXL Produkte)

Synonyme: JTK11 Protein, UFO Protein, AI323647 Protein, Ark Protein, Tyro7 Protein, Ufo Protein, AXL receptor tyrosine kinase Protein, Axl receptor tyrosine kinase Protein, AXL Protein, Axl Protein

Hintergrund: Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Molekulargewicht: 46.3 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.

UniProt: A0A1D5Q330

Pathways: RTK Signalweg, Cellular Response to Molecule of Bacterial Origin