APOE Protein (AA 19-317) (Fc Tag)
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- Target Alle APOE Proteine anzeigen
- APOE (Apolipoprotein E (APOE))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 19-317
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses APOE Protein ist gelabelt mit Fc Tag.
- Verwendungszweck
- Human APOE4/Apolipoprotein E Protein
- Sequenz
- Lys19-His317
- Produktmerkmale
- Recombinant Human APOE4/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Lys19-His317.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 11.87 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Apolipoprotein E (APOE) (AA 19-311) protein (Fc Tag)
APOE Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apolipoprotein E (APOE) (AA 19-311) protein (His tag)APOE Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Apolipoprotein E (APOE) (AA 19-311) protein (His tag)APOE Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Apolipoprotein E (APOE) (AA 19-317) protein (His tag)Crystallography grade APOE Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Apolipoprotein E (APOE) protein (Myc-DYKDDDDK Tag)APOE Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 2 mM DTT, 5 mM CHAPS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Konservierungsmittel
- Dithiothreitol (DTT)
- Vorsichtsmaßnahmen
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- APOE (Apolipoprotein E (APOE))
- Andere Bezeichnung
- APOE4 (APOE Produkte)
- Synonyme
- ad2 Protein, apoprotein Protein, im:7036787 Protein, wu:fb69a05 Protein, zgc:110064 Protein, apoe Protein, AI255918 Protein, AD2 Protein, LDLCQ5 Protein, LPG Protein, APOEA Protein, Apo-E Protein, apolipoprotein E Protein, apolipoprotein Ea Protein, apoe Protein, apoea Protein, Apoe Protein, APOE Protein
- Hintergrund
- Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
- Molekulargewicht
- 61.6 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.
- Pathways
- Regulation of Cell Size, Lipid Metabolism
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