DLL4 Protein (His tag)
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- Target Alle DLL4 Proteine anzeigen
- DLL4 (delta-Like 4 (DLL4))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses DLL4 Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Mouse DLL4 Protein (His Tag)(Active)
- Sequenz
- Met 1-Pro 525
- Produktmerkmale
- A DNA sequence encoding the extracellular domain of mouse DLL4 (NP_062327.2) (Met 1-Pro 525) was expressed, with a C-terminal polyhistidine tag.
- Reinheit
- > 96 % as determined by SDS-PAGE
- Endotoxin-Niveau
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA. Immobilized mouse DLL4-his at 10 μg/mL (100 μL/well) can bind biotinylated mouse NOTCH1-his. The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2-3 μg/mL.
- Top Product
- Discover our top product DLL4 Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- DLL4 (delta-Like 4 (DLL4))
- Andere Bezeichnung
- DLL4 (DLL4 Produkte)
- Synonyme
- hdelta2 Protein, Delta4 Protein, si:ch211-244p18.5 Protein, zgc:136596 Protein, delta like canonical Notch ligand 4 Protein, delta-like 4 (Drosophila) Protein, DLL4 Protein, Dll4 Protein, dll4 Protein
- Hintergrund
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Background: Delta-like protein 4 (DLL4, Delta4), a type I membrane-bound Notch ligand, is one of five known Notch ligands in mammals and interacts predominantly with Notch 1, which has a key role in vascular development. Recent studies yield substantial insights into the role of DLL4 in angiogenesis. DLL4 is induced by vascular endothelial growth factor (VEGF) and acts downstream of VEGF as a 'brake' on VEGF-induced vessel growth, forming an autoregulatory negative feedback loop inactivating VEGF. DLL4 is downstream of VEGF signaling and its activation triggers a negative feedback that restrains the effects of VEGF. Attenuation of DLL4/Notch signaling results in chaotic vascular network with excessive branching and sprouting. DLL4 is widely distributed in tissues other than vessels including many malignancies. Furthermore, the molecule is internalized on binding its receptor and often transported to the nucleus. In pathological conditions, such as cancer, DLL4 is up-regulated strongly in the tumour vasculature. Blockade of DLL4-mediated Notch signaling strikingly increases nonproductive angiogenesis, but significantly inhibits tumor growth in preclinical mouse models. In preclinical studies, blocking of DLL4/Notch signaling is associated with a paradoxical increase in tumor vessel density, yet causes marked growth inhibition due to functionally defective vasculature. Thus, DLL4 blockade holds promise as an additional strategy for angiogenesis-based cancer therapy.
Synonym: Delta4
- Molekulargewicht
- 55.7 kDa
- NCBI Accession
- NP_062327
- Pathways
- Notch Signalweg
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