CDKN2D Protein (GST tag)

Produktnummer ABIN7317361

Produktdetails

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CDKN2D Protein ist gelabelt mit GST tag.

Verwendungszweck: Recombinant Human CDKN2D/p19ink4d Protein (GST Tag)(Active)

Sequenz: Met 10Leu 166

Produktmerkmale: A DNA sequence encoding the human CDKN2D (P55273) (Met 10Leu 166) was fused with the GST tag at the N-terminus.

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Immobilized human GST-CDKN2D at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 0.52-1.2 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CDKN2D (Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))

Andere Bezeichnung: CDKN2D/p19ink4d (CDKN2D Produkte)

Synonyme: ink4d Protein, p19 Protein, p19-ink4d Protein, CDKN2D Protein, ank2 Protein, INK4D Protein, p19-INK4D Protein, INK4d Protein, p19INK4d Protein, cyclin-dependent kinase inhibitor 2D S homeolog Protein, cyclin dependent kinase inhibitor 2D Protein, cyclin-dependent kinase inhibitor 2D Protein, Cyclin-dependent kinase 4 inhibitor D Protein, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) Protein, similar to cyclin-dependent kinase inhibitor 2D Protein, cdkn2d.S Protein, CDKN2D Protein, cdkn2d Protein, cdn2d Protein, Cdkn2d Protein

Hintergrund:

Background: Cyclin-dependent kinase inhibitor 2D(also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19(Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers. 

Synonym: INK4D;p19;p19-INK4D

Molekulargewicht: 44.9 kDa

UniProt: P55273

Pathways: Zellzyklus, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling