SARS-CoV-2 Spike Protein (B.1.617.2 - delta, BA.1 - Omicron, Trimer) (His tag)

Produktnummer ABIN7121374

Produktdetails

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Proteineigenschaft: B.1.617.2 - delta, BA.1 - Omicron, Trimer

Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 XD

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His tag.

Verwendungszweck: SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (MALS verified)

Sequenz: Val 16 - Pro 1213

Spezifität: SARS-CoV-2 Spike Trimer (XD/BA.1 x AY.4 recombinant)

Produktmerkmale: SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (SPN-C5226) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P). The spike mutations are predicted as the SARS-CoV-2 variant (Pango lineage: XD). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Güteklasse: MALS verified

Anwendungsinformationen

Applikationshinweise: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.5 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produkte

Synonyme: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Hintergrund: Synonyms: Spike,S protein,Spike glycoprotein,S glycoprotein,
Description: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 138.5 kDa