SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag)

Produktnummer ABIN6953302

Produktdetails

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Proteineigenschaft: Super Stable Trimer

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His tag.

Applikation: ELISA

Verwendungszweck: SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)

Sequenz: AA 16-1213

Produktmerkmale: SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Reinheit: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: This product is specifically designed for antibody drug development.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution.

Haltbarkeit: 12 months

Referenzen

Yokoyama, Ichiki: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produkte

Synonyme: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 138.0 kDa