SARS-CoV-2 Spike Protein (Trimer) (rho-1D4 tag)

Produktnummer ABIN6952670

Produktdetails

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Proteineigenschaft: Trimer

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Verwendungszweck: Trimeric, full length Cov-2 spike protein for assay development (""Antibody tests"")

Spezifität:

• single span transmembrane membrane protein (aa 1-1273)
• Furin cleavage site ""RRAR"" mutated to ""GSAS""
• trimerization (3 x 142 kDa) shown on native PAGE
• expressed in Expi293™ cells
• C-terminal Rho1D4 tag for affinity purification
• Solubilization and stabilization in LMNG detergent
• 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Full length SARS Cov-2 spike protein expressed in Expi293™ cells to assure native state glycosylation.
• Purification by a multi-step, protein-specific protocol to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: The protein is purified from the cleared cell lysate using Rho1D4 capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Protein is endotoxin-free.

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Produkte

Synonyme: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund: The spike glycoprotein exists as a homotrimeric fusion protein. Each of the trimers contains 66 glycosylation sites for host-derived N-linked glycans. Accordingly, expression of this primary target for SARS-CoV-2 vaccine development in an appropriate, human expression system is of utmost importance. Prior to ACE2 binding, each monomer in the prefusion complex contains an S1 ectodomain including the receptor binding domain (RBD) and an S2 endodomain harboring a transmembrane domain. In the predominant state of the trimer, one of the RBDs is in an “up” position whereas the other two are in a “down” position. Interaction of S-protein and ACE2 only takes place with the RBD in the “up” position. Receptor binding triggers a structural change that leads to separation of the S1 and S2 subunits.

Molekulargewicht: 3 x 142 kDa

Gen-ID: 43740568

UniProt: P0DTC2