SARS-CoV-2 Spike S1 Protein (His tag,Fc Tag)

Produktnummer ABIN6952732

Produktdetails

Target: SARS-CoV-2 Spike S1

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His tag,Fc Tag.

Applikation: SDS-PAGE (SDS), Functional Studies (Func)

Sequenz: VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PR

Spezifität: Val11-Arg682

Produktmerkmale: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6xHis tag at the C-terminus.

Reinheit: > 90 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC₅₀ of SARS-COV-2 Spike S1 is 6.79 ng/mL.< /br>2.Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Konzentration: 1 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Antigendetails

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Produkte

Synonyme: E2 Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund:

Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

Name: Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein

Gen-ID: 43740568

UniProt: P0DTC2