RPA2 Protein (AA 1-270) (His tag)

Produktnummer ABIN7279229

Produktdetails

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-270

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RPA2 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: RPA2, 1-270aa, Human, His tag, E.coli

Reinheit: > 85 % by SDS – PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Lagerung: 4 °C

Antigendetails

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA2 (RPA2 Produkte)

Synonyme: REPA2 Protein, RPA32 Protein, 153194_at Protein, CG9273 Protein, Dmel\\CG9273 Protein, dRPA2 Protein, rpa Protein, zgc:109822 Protein, ik:tdsubc_2g1 Protein, xx:tdsubc_2g1 Protein, repa2 Protein, rpa32 Protein, RPA2 Protein, GB13976 Protein, LOC100230445 Protein, AA409079 Protein, AI325195 Protein, AU020965 Protein, RPA34 Protein, Rf-A2 Protein, replication protein A2 Protein, Replication protein A2 Protein, replication protein A2, 32kDa Protein, replication protein A 32 kDa subunit Protein, RPA2 Protein, Rpa2 Protein, rpa2 Protein, LOC725418 Protein, RPA2_2 Protein

Hintergrund: Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. The C-terminus of RPA2 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, this protein binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3. Recombinant human RPA2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Replication protein A 32 kDa subunit , REPA2, RPA32, RPA32, RP-A p32, RP-A p34. NCBI no.: NP_002937

Molekulargewicht: 31.7 kDa (293aa) confirmed by MALDI-TOF

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA