RPA2 Protein (AA 1-267, partial) (GST tag)

Produktnummer ABIN7479332

Produktdetails

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-267, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RPA2 Protein ist gelabelt mit GST tag.

Applikation: ELISA

Sequenz: MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT ISQLLSATLV DEVFRIGNVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA PMDVRQWVDT DDTSSENTVV PPETYVKVAG HLRSFQNKKS LVAFKIMPLE DMNEFTTHIL EVINAHMVLS KANSQPSAGR APISNPGMSE AGNFGGNSFM PANGLTVAQN QVLNLIKACP RPEGLNFQDL KNQLKHMSVS SIKQAVDFLS NEGHIYSTVD DDHFKST

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Antigendetails

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: Replication protein A 32 kDa subunit protein (RPA2 Produkte)

Synonyme: REPA2 Protein, RPA32 Protein, 153194_at Protein, CG9273 Protein, Dmel\\CG9273 Protein, dRPA2 Protein, rpa Protein, zgc:109822 Protein, ik:tdsubc_2g1 Protein, xx:tdsubc_2g1 Protein, repa2 Protein, rpa32 Protein, RPA2 Protein, GB13976 Protein, LOC100230445 Protein, AA409079 Protein, AI325195 Protein, AU020965 Protein, RPA34 Protein, Rf-A2 Protein, replication protein A2 Protein, Replication protein A2 Protein, replication protein A2, 32kDa Protein, replication protein A 32 kDa subunit Protein, RPA2 Protein, Rpa2 Protein, rpa2 Protein, LOC725418 Protein, RPA2_2 Protein

Hintergrund: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Ref.9 Ref.13 Ref.14 Ref.15 Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Molekulargewicht: 56.3 kD

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA