PKLR Protein (AA 47-574) (His tag)

Produktnummer ABIN667822

Produktdetails

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Protein-Typ: Recombinant

Proteineigenschaft: AA 47-574

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PKLR Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: PKLR, 47-574aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Lagerung: 4 °C

Antigendetails

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Andere Bezeichnung: PKLR (PKLR Produkte)

Synonyme: PK1 Protein, PKL Protein, PKR Protein, PKRL Protein, RPK Protein, Pklg Protein, wu:fd15e01 Protein, wu:fi37e08 Protein, pk1 Protein, PKLR Protein, Pk-1 Protein, Pk1 Protein, R-PK Protein, pklr Protein, pyruvate kinase L/R Protein, pyruvate kinase, liver and RBC Protein, pyruvate kinase, liver and RBC L homeolog Protein, pyruvate kinase liver and red blood cell Protein, pyruvate kinase PKLR-like Protein, PKLR Protein, Pklr Protein, pklr Protein, pklr.L Protein, LOC100621940 Protein

Hintergrund: PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK. NCBI no.: NP_000289

Molekulargewicht: 59.2 kDa (549aa), confirmed by MALDI-TOF

Pathways: Ribonucleoside Biosynthetic Process