POLB Protein (AA 1-335) (His tag)

Produktnummer ABIN667538

Produktdetails

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-335

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLB Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: POLB, 1-335aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Lagerung: 4 °C

Antigendetails

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Andere Bezeichnung: POLB (POLB Produkte)

Synonyme: wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein

Hintergrund: DNA polymerase beta, also known as POLB, belongs to the DNA polymerase type-X family. In eukaryotic cells, DNA polymerase beta (POLB) performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance. POLB has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. Overexpression of POLB, as found in some human tumors, could confer an increase in spontaneous mutagenesis. Recombinant human POLB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: DNA polymerase beta, DNA directed DNA polymerase beta, DNA pol beta, DNA polymerase beta, DNA polymerase beta subunit, MGC125976, Pol B, Pol beta, PolB, Polymerase (DNA directed) beta. NCBI no.: NP_002681

Molekulargewicht: 40.3 kDa (355aa), confirmed by MALDI-TOF

Pathways: DNA Reparatur