POLB Protein (His tag)
-
- Target Alle POLB Proteine anzeigen
- POLB (Polymerase (DNA Directed), beta (POLB))
- Protein-Typ
- Recombinant
-
Spezies
- Human
-
Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses POLB Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Human DNA Polymerase β/POLB Protein (His Tag)
- Sequenz
- Ser2-Glu335
- Produktmerkmale
- Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.
- Reinheit
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin-Niveau
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product POLB Protein
-
Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His tag)
POLB Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 224-333) protein (GST tag)POLB Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (Strep Tag)Crystallography grade POLB Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (DNA Directed), beta (POLB) (Active) proteinPOLB Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure by SDS-PAGE analysis Func Active
-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
-
- Target
- POLB (Polymerase (DNA Directed), beta (POLB))
- Andere Bezeichnung
- DNA Polymerase beta/POLB (POLB Produkte)
- Synonyme
- wu:fa97a05 Protein, zgc:109924 Protein, POLB Protein, DDBDRAFT_0186152 Protein, DDBDRAFT_0215784 Protein, DDBDRAFT_0232376 Protein, DDB_0186152 Protein, DDB_0215784 Protein, DDB_0232376 Protein, A430088C08Rik Protein, polymerase (DNA directed), beta Protein, DNA polymerase beta Protein, DNA polymerase X family protein Protein, polymerase (DNA directed), beta S homeolog Protein, polb Protein, POLB Protein, polB Protein, polb.S Protein, Polb Protein
- Hintergrund
-
Background: Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
Synonym: DNA Polymerase Beta, POLB
- Molekulargewicht
- 39.2 kDa
- UniProt
- P06746
- Pathways
- DNA Reparatur
-
