NEDD8 Protein (AA 1-76) (His tag)

Produktnummer ABIN666733

Produktdetails

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-76

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NEDD8 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: NEDD8, 1-76 aa, Human, His tagged, Recombinant, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol

Lagerung: 4 °C

Antigendetails

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Andere Bezeichnung: NEDD8 (NEDD8 Produkte)

Synonyme: NEDD-8 Protein, Rub1 Protein, CG10679 Protein, Dmel\\CG10679 Protein, NEDD8 Protein, dNEDD8 Protein, nedd8 Protein, ubiquitin Protein, si:zc14a17.5 Protein, GB17785 Protein, DDBDRAFT_0218116 Protein, DDBDRAFT_0238041 Protein, DDB_0218116 Protein, DDB_0238041 Protein, nedd8.S Protein, neural precursor cell expressed, developmentally down-regulated 8 Protein, neural precursor cell expressed, developmentally down-regulated gene 8 Protein, CG10679 gene product from transcript CG10679-RB Protein, Nedd8 Protein, ubiquitin-like protein Nedd8 Protein, neural precursor cell expressed, developmentally down-regulated 8 L homeolog Protein, NEDD8 Protein, Nedd8 Protein, nedd8 Protein, nedd8.L Protein

Hintergrund: NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer. Synonyms: Ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, Ubiquitin like protein Nedd 8, Ubiquitin like protein Nedd8. NCBI no.: NP_006147

Molekulargewicht: 12.8 kDa (113 aa), confirmed by MALDI-TOF.

Pathways: Ubiquitin Proteasome Pathway