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TIMD4 Protein (Fc Tag,Biotin)

TIMD4 Spezies: Human, Maus Wirt: CHO Cells Recombinant >95 % (SDS-PAGE)
Produktnummer ABIN6253504
  • Target Alle TIMD4 Proteine anzeigen
    TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))
    Protein-Typ
    Recombinant
    Spezies
    • 8
    • 6
    • 1
    Human, Maus
    Quelle
    • 6
    • 4
    • 2
    • 1
    • 1
    CHO Cells
    Aufreinigungstag / Konjugat
    Dieses TIMD4 Protein ist gelabelt mit Fc Tag,Biotin.
    Verwendungszweck
    Tim-4 (mouse):Fc (human) (rec.) (Biotin)
    Spezifität
    The extracellular domain of mouse Tim-4 (aa 22-279) is fused to the N-terminus of the Fc region of human IgG1.
    Produktmerkmale
    Protein. The extracellular domain of mouse Tim-4 (aa 22-279) is fused to the N-terminus of the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.05EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). Extracellular vesicles (EVs) are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes (30-100nm), microvesicles (100-1000nm) and apoptotic bodies (>1000nm). EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs. Therefore, developing ideal methods for isolating and quantifying EVs is an active area of research. EVs express phosphatidylserine (PS) on their outer lipid bilayer. Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4) is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and TIM family. Tim-4 contains one Ig-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. Tim-4 plays an important role in the proliferation of T helper type 2 (Th2) cells. Tim-4 binds to phosphatidylserine (PS) on the surface of apoptotic cells in a calcium-dependent manner and mediates phagocytosis of apoptotic cells. EV membranes are rich in phosphatidylserine (PS) and Tim-4 binds to PS on the surface of EVs. A new protocol from the group of Prof. Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner. This new protocol could replace ultracentrifugation, that is the most commonly used method for purifying EVs. This new Tim-4-dependent method gives good yield, high purity and allows isolation of all populations of EVs compared to other approaches (ultracentrifugation, PEG precipitation or selected antibodies immunoprecipitation). See Reference 2 for a complete protocol (Download available).
    Reinheit
    >95 % (SDS-PAGE)
    Endotoxin-Niveau
    <0.05EU/μg protein (LAL test, Lonza).
    Biological Activity Comment
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The Tim-4 (mouse):Fc (human) (rec.) (Biotin) is used with magnetic beads to isolate extracellular vesicles. 120ng of the protein is sufficient to isolate 1010 particles in a calcium-dependent manner.
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  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Konzentration
    Lot specific
    Buffer
    Lyophilized from 0.2μm-filtered solution in PBS.
    Handhabung
    Avoid freeze/thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung

    Short Term Storage: +4°C

    Long Term Storage: -20°C

    Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

  • Target
    TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))
    Andere Bezeichnung
    Tim-4 (TIMD4 Produkte)
    Synonyme
    RGD1564516 Protein, MGC99322 Protein, TIMD4 Protein, B430010N18Rik Protein, TIM-4 Protein, Tim4 Protein, SMUCKLER Protein, TIM4 Protein, T-cell immunoglobulin and mucin domain containing 4 Protein, T-cell immunoglobulin and mucin domain containing 4 L homeolog Protein, T cell immunoglobulin and mucin domain containing 4 Protein, Timd4 Protein, TIMD4 Protein, timd4.L Protein
    Hintergrund

    Alternate Names/Synonyms: TIM4, TIMD4, T Cell Immunoglobulin and Mucin Domain-containing Protein 4

    Product Description: Extracellular vesicles (EVs) are released by a variety of cells into the cellular microenvironment and have the natural ability of delivering different cargos and carry bioactive molecules such as non-coding RNA, miRNAs, genomic DNA, lipids, growth factors and signaling molecules. EVs can be divided into exosomes (30-100nm), microvesicles (100-1000nm) and apoptotic bodies (>1000nm). EVs play substantial roles not only in the regulation of normal physiological processes but also in disease pathogenesis and their cargo reflects the status of parental cells at the time of secretion. Various studies are currently being conducted to develop therapeutic and diagnostic methods targeting or utilizing EVs. Therefore, developing ideal methods for isolating and quantifying EVs is an active area of research. EVs express phosphatidylserine (PS) on their outer lipid bilayer. Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4) is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and TIM family. Tim-4 contains one Ig-like V-type (immunoglobulin-like) domain. It is expressed on dendritic cells and macrophages. Tim-4 plays an important role in the proliferation of T helper type 2 (Th2) cells. Tim-4 binds to phosphatidylserine (PS) on the surface of apoptotic cells in a calcium-dependent manner and mediates phagocytosis of apoptotic cells. EV membranes are rich in phosphatidylserine (PS) and Tim-4 binds to PS on the surface of EVs. A new protocol from the group of Prof. Rikinari Hanayama describes an affinity-based method for isolating EVs using streptavidin magnetic beads conjugated with Tim-4-biotin to capture EVs in a calcium-dependent manner. This new protocol could replace ultracentrifugation, that is the most commonly used method for purifying EVs. This new Tim-4-dependent method gives good yield, high purity and allows isolation of all populations of EVs compared to other approaches (ultracentrifugation, PEG precipitation or selected antibodies immunoprecipitation). See Reference 2 for a complete protocol (Download available).

    NCBI Accession
    NP_848874
    Pathways
    Cancer Immune Checkpoints
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