PCBP1 Protein (AA 1-163) (His tag)

Produktnummer ABIN5853223

Produktdetails

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-163

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PCBP1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Andere Bezeichnung: PCBP1 (PCBP1 Produkte)

Synonyme: PCBP1 Protein, HNRPE1 Protein, HNRPX Protein, hnRNP-E1 Protein, hnRNP-X Protein, hnRNP E1 Protein, WBP17 Protein, [a]CP-1 Protein, alphaCP-1 Protein, RGD1561319 Protein, poly(rC) binding protein 1 Protein, poly(rC) binding protein 1 L homeolog Protein, poly(rC)-binding protein 1 Protein, PCBP1 Protein, pcbp1.L Protein, LOC100388387 Protein, Pcbp1 Protein

Hintergrund: PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 19.6 kDa (186aa) confirmed by MALDI-TOF

NCBI Accession: NP_006187

UniProt: Q15365