IL-5 Protein (AA 20-134) (His tag)

Produktnummer ABIN5777626

Produktdetails

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-134

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses IL-5 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: ADPIPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIESHH HHHH

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 1.5 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL5 (IL5 Produkte)

Synonyme: EDF Protein, IL-5 Protein, TRF Protein, Il-5 Protein, IL5 Protein, interleukin 5 Protein, IL5 Protein, Il5 Protein

Hintergrund: IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant human IL5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 14.2kDa (124aa) 13.5-18kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_000870

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour