IL-5 Protein (AA 20-134) (His-Avi Tag,Biotin)

Produktnummer ABIN7274955

Produktdetails

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-134

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-5 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human IL-5 Protein

Sequenz: Ile20-Ser134

Produktmerkmale: Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 39ng/ml determined by ELISA. The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL-5 (IL5 Produkte)

Synonyme: EDF Protein, IL-5 Protein, TRF Protein, Il-5 Protein, IL5 Protein, interleukin 5 Protein, IL5 Protein, Il5 Protein

Hintergrund: IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Molekulargewicht: 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour