XRCC6 Protein (AA 6-222, partial) (His tag)

Produktnummer ABIN5712169

Produktdetails

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Protein-Typ: Recombinant

Proteineigenschaft: partial, AA 6-222

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses XRCC6 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: SYYKTEGDEE AEEEQEENLE ASGDYKYSGR DSLIFLVDAS KAMFESQSED ELTPFDMSIQ CIQSVYISKI ISSDRDLLAV VFYGTEKDKN SVNFKNIYVL QELDNPGAKR ILELDQFKGQ QGQKRFQDMM GHGSDYSLSE VLWVCANLFS DVQFKMSHKR IMLFTNEDNP HGNDSAKASR ARTKAGDLRD TGIFLDLMHL KKPGGFDISL FYRDIIS

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: XRCC6 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))

Andere Bezeichnung: XRCC6 (XRCC6 Produkte)

Synonyme: CTC75 Protein, CTCBF Protein, G22P1 Protein, KU70 Protein, ML8 Protein, TLAA Protein, 70kDa Protein, G22p1 Protein, Ku70 Protein, Kup70 Protein, X-ray repair cross complementing 6 Protein, ATP-dependent DNA helicase II, 70 kDa subunit Protein, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog Protein, X-ray repair complementing defective repair in Chinese hamster cells 6 Protein, XRCC6 Protein, Bm1_41430 Protein, xrcc6.L Protein, Xrcc6 Protein

Hintergrund: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing th together. The assbly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription

Molekulargewicht: 28.9 kDa

UniProt: P12956

Pathways: DNA Reparatur