LBP Protein (His tag)

Produktnummer ABIN5663348

Produktdetails

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: CHO Cells

Aufreinigungstag / Konjugat: Dieses LBP Protein ist gelabelt mit His tag.

Applikation: ELISA, Agonist (Agon), Flow Cytometry (FACS)

Sequenz: MKAGTGPLLS TLLGLLFLSI QGTGGVNPGV VARITDKGLA YAAKEGLVAL QRELYKITLP DFSGDFKIKA VGRGQYEFHS LEIQNCELRG SSLKLLPGQG LSLAISDSSI GVRGKWKVRK SFLKLHGSFD LDVKGVTISV DLLLGMDPSG RPTVSASGCS SRICDLDVHI SGNVGWLLNL FHNQIESKLQ KVLENKVCEM IQKSVTSDLQ PYLQTLPVTA EIDNVLGIDY SLVAAPQAKA QVLDVMFKGE IFNRNHRSPV FSITDDMLPH DSGIRLNTKA FRPFTPQIYK KYPDMKLELL GTVVSAPILN ATPTPTMSLP EDSKQMVYFA ISDYAFNIAS RVYHQAGYLN VSPGNLSLAP QMEIEGFVIL PTSAREPVFR LGVVTNVFAS LTFNNSKVTG MLHPDKAQVR LIESKVGMFN VNLFQAFLNY YLLNSLYPDV NAELAQGFPL PLPRHIQLHD LDFQIRKDFL YLGANVQYMR VHHHHHH

Produktmerkmale: Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off

Aufreinigung: Affinity chromatography by His-Tag

Reinheit: >95 % by SDS-PAGE

Endotoxin-Niveau: <1 EU/μg of recombinant mouse LBP as determined by LAL method

Biological Activity Comment: up to 2 μg/ ml LBP mediates binding of FITC-LPS (0.5μg/ml) to CD14+CHO transfectants (2 x 106/ml)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Konzentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.6

Konservierungsmittel: Without preservative

Lagerung: RT,-20 °C,-80 °C

Informationen zur Lagerung:

Storage: -20°C or -70°C

Storage/Stability: The lyophilized product thought stable at room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Use reconstituted protein immediately or stored in undiluted working aliquots at -20oC. For long-term storage, we recommend add of carrier protein (10% BSA). Repeated freezing and thawing should be avoided!!!The LBP will be precipitate by rapid changes of pH or temperature. Best working temperature is ice bath

Haltbarkeit: 12 months

Antigendetails

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Andere Bezeichnung: LBP (LBP Produkte)

Synonyme: BPIFD2 Protein, Bpifd2 Protein, Ly88 Protein, LPSBP Protein, LBP Protein, lipopolysaccharide binding protein Protein, lipopolysaccharide-binding protein Protein, LBP Protein, Lbp Protein, LOC100472839 Protein

Hintergrund: Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes.Recombinant LBP produced from mouse LBP transfected CHO-cells in serum free medium. For transfection, we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection, the complete mouse LBP cDNA amplified by PCR and cloned into expression vector p-POL-DHFR.Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Sequence similarities belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family.

Molekulargewicht: ~58kDa

Gen-ID: 16803

UniProt: Q61805

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process