IDO1 Protein (AA 1-403) (His tag)

Produktnummer ABIN5564386

Produktdetails

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-403

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses IDO1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Kreuzreaktivität: Human

Produktmerkmale: Human IDO (aa 1-403) is fused at the N-terminus to a His-tag.

Reinheit: >90 % (SDS-PAGE)

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: <0.1EU/μg purified protein (LAL test, Lonza).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Specific Activity: >100'000U/mg protein with L-tryptophan as substrate (activity assay with catalase). One unit is defined as the amount of enzyme that produces 1nmol of N-formylkynurenine (NFK) per hour. For more information see Activity Test Protocol.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.2μm-filtered solution in 10 mM Tris, pH 7.5, containing 100 mM NaCl and 20 % glycerol.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 1 year after receipt when stored at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Andere Bezeichnung: IDO (IDO1 Produkte)

Synonyme: INDO Protein, ido Protein, indo Protein, IDO Protein, cd107b Protein, IDO1 Protein, IDO-1 Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, indoleamine 2,3-dioxygenase 1 L homeolog Protein, indoleamine 2,3-dioxygenase 1-like Protein, IDO1 Protein, ido1.L Protein, ido1 Protein, Ido1 Protein, LOC100050681 Protein

Hintergrund: IDO is a heme enzyme that catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway, causing depletion of tryptophan which can lead to halted growth of microbes as well as T cells. IDO is an immune checkpoint protein, thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation and antioxidant activity. Cancer cells are able to evade the immune system is by hijacking the checkpoint proteins. Increased IDO protein levels drive growth arrest and apoptosis of the effector T cells, a group of immune cells that mediate the immune system's ability to destroy pathogens. By reducing the number of effector T cells, IDO overexpression prevents the immune system from effectively destroying cancer cells. IDO overexpression has been observed in a wide range of human cancers such as prostatic, colorectal, pancreatic, cervical, gastric, ovarian, head or lung cancer. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Biological active IDO enzyme can be used for screening of IDO inhibitors, which have shown promising antitumor activity and appear to be synergistic in combination with chemotherapy and other forms of immunotherapy.

Molekulargewicht: ~47kDa (SDS-PAGE)

UniProt: P14902

Pathways: Activated T Cell Proliferation