HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Spezies
Human
Quelle
Hi-5 Cells
Aufreinigungstag / Konjugat
Dieses HVEM Protein ist gelabelt mit Fc Tag.
Applikation
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human TNFRSF14 / HVEM protein expressed in Hi-5 Insect cells.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin-Niveau
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by its ability to neutralize 0.25 ng/ml of hTNF&beta induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/mL of HVEM-Fc.
TNFRSF14
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
Active
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Handhabung
Resuspend the protein in the desired concentration in proper buffer
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target
HVEM (TNFRSF14)
(Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.