HVEM Protein (AA 39-202) (Fc Tag)

Produktnummer ABIN7274832

Produktdetails

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Protein-Typ: Recombinant

Proteineigenschaft: AA 39-202

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses HVEM Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human HVEM/TNFRSF14 Protein

Sequenz: Leu39-Val202

Produktmerkmale: Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.2μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))

Andere Bezeichnung: HVEM (TNFRSF14 Produkte)

Synonyme: ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein

Substanzklasse: Viral Protein

Hintergrund: Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

Molekulargewicht: 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints