BTLA Protein (AA 31-134) (Fc Tag,AVI tag,Biotin)

Produktnummer ABIN4949010

Produktdetails

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 31-134

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses BTLA Protein ist gelabelt mit Fc Tag,AVI tag,Biotin.

Applikation: Functional Studies (Func)

Marke: MABSol®,PrecisionAvi

Sequenz: AA 31-134

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Andere Bezeichnung: BTLA (BTLA Produkte)

Synonyme: BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein

Hintergrund: B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

Molekulargewicht: 40.5 kDa

UniProt: Q7Z6A9

Pathways: Cancer Immune Checkpoints