BTLA Protein (His-Avi Tag,Biotin)
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- Target Alle BTLA Proteine anzeigen
- BTLA (B and T Lymphocyte Associated (BTLA))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses BTLA Protein ist gelabelt mit His-Avi Tag,Biotin.
- Sequenz
- Lys31-Ser150
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-BTLA Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
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B and T Lymphocyte Associated (BTLA) (AA 31-150) protein (Fc Tag)
BTLA Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) (AA 31-150) protein (His-Avi Tag)BTLA Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc-His Tag)BTLA Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
B and T Lymphocyte Associated (BTLA) (AA 30-176) protein (His tag)BTLA Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
B and T Lymphocyte Associated (BTLA) protein (mFc Tag)BTLA Spezies: Human Wirt: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
B and T Lymphocyte Associated (BTLA) (AA 190-289) protein (GST tag)BTLA Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
B and T Lymphocyte Associated (BTLA) (AA 25-289) protein (His tag)BTLA Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant SDS, Func
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag,ECD)BTLA Spezies: Ratte Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (Active) protein (Fc Tag)BTLA Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
B and T Lymphocyte Associated (BTLA) (AA 179-289) protein (His tag)Crystallography grade BTLA Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- BTLA (B and T Lymphocyte Associated (BTLA))
- Andere Bezeichnung
- BTLA (BTLA Produkte)
- Synonyme
- BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein
- Hintergrund
- CD272, BTLA, BTLA1, FLJ16065, MGC129743,B- and T-lymphocyte attenuator (BTLA, CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
- Molekulargewicht
- 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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