MALT1 Protein (AA 2-832) (His tag)

Produktnummer ABIN3134944

Produktdetails

Target: MALT1 (Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1 (MALT1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-832

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses MALT1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SLWGQPLQAS PPLAVRQPPT ASSGPSTSPP AGATLNRLPE PLLRRLSESL DRAPEGRGWR QLAELAGSRG RLRLSGLDLE QCSLKVLEPE GSPSLCLLKL MGEKGCTVTE LSDFLQALEH TEVLPLLNPP GLKITVNPES KAVLAGQFVK LCCRATGHPF VQYQWFKMNK EIPYGNSSEL VFNTVHVKDA GFYVCRVNNS STFEFSQWSQ LDVCDVAEVT DSFQGSMDGI SESRLQICVE PRSQRLVPGS MLLLQCVAIG SPMPHYQWFK DESPLTHETK KHYTVPYVDI EHEGTYWCHV YNDRDSQDSK KAEVTIGRTD EAVECTEDEL NNLGHPDNKE QTGQPLAKDK VALLIGNMSY WEHPKLKAPL VDVYELTNLL RQLDFKVVSL LDLTEYEMCN AVDEFLLLLD KGVYGLLYYA GHGYENFGNS FMVPVDAPNP YRSENCLCVQ NILKLMQEKE TGLNVFLLDM CRKRNDYDDT IPILDALKVT ANIVFGYATC QGAEAFEIQH SGLANGIFMK FLKDRLLEDK KITVLLDEVA EDMGKCHLTK GRQALEIRSS LSEKRALTDP VQGAPCSAEA LVRNLQWAKA HELPESMCLK FQCGVHIQLG FAAEFSNVMI IYTSIVHKPP EIIMCDAYVT DFPLDLDIDP KHANKGTPEE TGSYLVSKDL PKHCLYTRLS SLQKLKEHLI FTVCLSYQYS GLEDTVEEKQ EVNVGKPLIA KLDMHRGLGR KTCFQACRMP DEPYHSSTST SAGAGHFHSS QDSFHDVYHS HLGNADSGMP PDRCHCSRTP HTFISNYPPH HYCQFGRSNV PVETTDEMPF SFSDRLMISE N
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Malt1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MALT1 (Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1 (MALT1))

Andere Bezeichnung: Malt1 (MALT1 Produkte)

Synonyme: malt1 Protein, MALT1 Protein, mlt Protein, mlt1 Protein, MLT Protein, MLT1 Protein, A630046N12 Protein, D430033E09Rik Protein, MALT paracaspase 2 Protein, MALT1 paracaspase Protein, MALT1 paracaspase S homeolog Protein, paracaspase 3 Protein, malt2 Protein, MALT1 Protein, malt1.S Protein, Malt1 Protein, PCASP3 Protein

Hintergrund: Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity (By similarity). MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion (By similarity). {ECO:0000250}.

Molekulargewicht: 94.0 kDa Including tag.

UniProt: Q2TBA3

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine