TNFSF12
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, PI, AbP, STD
Active
TWEAK (TNFSF12)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Spezies
Human
Quelle
Escherichia coli (E. coli)
Applikation
Functional Studies (Func), Protein Interaction (PI), Antibody Production (AbP), Standard (STD)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human TWEAK / TNFSF12 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin-Niveau
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Assay #1: ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. ED50 for this effect is between 30-45 ng/ml.
TNFSF12
Spezies: Human
Wirt: CHO Cells
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Handhabung
Resuspend the protein in the desired concentration in proper buffer
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target
TWEAK (TNFSF12)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))
This gene encodes a member of the tumor necrosis factor superfamily. It encodes a hybrid protein composed of the cytoplasmic and transmembrane domains of family member 12 fused to the C-terminal domain of family member 13. The hybrid protein is membrane anchored and presents the receptor-binding domain of family member 13 at the cell surface. It stimulates cycling in T- and B-lymphoma cell lines.