PDK3 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2728576

Produktdetails

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PDK3 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human PDK3 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Andere Bezeichnung: Pdk3 (PDK3 Produkte)

Synonyme: pdk3 Protein, PDK3 Protein, 2610001M10Rik Protein, AI035637 Protein, zgc:158702 Protein, pyruvate dehydrogenase kinase 3 Protein, pyruvate dehydrogenase kinase, isozyme 3a Protein, pyruvate dehydrogenase kinase, isoenzyme 3 Protein, fragile site, 5-azacytidine type, common, fra(1)(q12) Protein, pyruvate dehydrogenase kinase, isozyme 3b Protein, PDK3 Protein, Pdk3 Protein, pdk3a Protein, pdk3 Protein, FRA1J Protein, pdk3b Protein

Hintergrund: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 46.8 kDa

NCBI Accession: NP_005382

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt