BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Protein-Typ
Recombinant
Biologische Aktivität
Active
Spezies
Maus
Quelle
CHO Cells
Applikation
Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human CD257 / BAFF protein expressed in CHO cells.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 95 % , as determined by Coomassie stained SDS-PAGE.
Endotoxin-Niveau
Less than 1 EU per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, determined by the dose dependent stimulation of B cells proliferation.
TNFSF13B
Spezies: Human
Wirt: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
> 50 μg/mL
Buffer
1 x PBS, pH 6.5
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Target
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))