BAFF Protein (AA 4-285) (His tag)

Produktnummer ABIN1019692

Produktdetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 4-285

Spezies: Human

Quelle: Tobacco (Nicotiana benthamiana)

Aufreinigungstag / Konjugat: Dieses BAFF Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), Cell Culture (CC)

Sequenz: HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL

Spezifität: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific BAFF antibody.

Produktmerkmale: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel transferred by electroblotting to a NC membrane and visualized by immune-detection with specific BAFF antibody.
Molecular formula: C830H1277N223O242S5.
Isoelectric Point: 63.
Extinction coefficient: E 0.1 % (1g/L) = 0.791 (A 280 nm).
This product contains no animal-derived components or impurities. Produced by transient expression of human

Aufreinigung: recombinant Human B lymphocyte activating factor (BAFF) in non-transgenic plants. BAFF contains a 10-His-tag at the N-terminal end and is purified by sequential chromatography (Affinity and Anionic exchange -FPLC).

Reinheit: > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Endotoxin-Niveau: < 0.04 EU/µg protein (LAL method)

Anwendungsinformationen

Kommentare:

Biological Activity: The activity is determined by dose-dependent response of proliferation Anti IgG M stimulated B cell from Human PBMC. Cell proliferation was measured by MTT method. The ED50 for this effect is typically < 10 ng/ml in presence of goat anti-human IgM (µ chain specific).
Recombinant human BAFF is a glycosylated polypeptide chain containing 151 amino acids (134-285 aa Q9Y275 (TN13B_HUMAN) fused to 10 His tag at N-terminal. rHuman BAFF has a molecular mass between 18 and 20kDa. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human B lymphocyte activating factor (BAFF) contains a 10-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal-derived components or impurities. Animal free product.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng/µL. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.

Buffer: 20 mM PBS buffer pH 7 and 0.2 M NaCl

Lagerung: 4 °C

Antigendetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Andere Bezeichnung: TNFSF13B (TNFSF13B Produkte)

Synonyme: BAFF Protein, BLYS Protein, CD257 Protein, DTL Protein, TALL-1 Protein, TALL1 Protein, THANK Protein, TNFSF20 Protein, ZTNF4 Protein, BAFF-R Protein, BAFFR Protein, BROMIX Protein, CD268 Protein, CVID4 Protein, prolixin Protein, TNFSF13B Protein, RGD1561519 Protein, RGD1560810 Protein, BLyS Protein, D8Ertd387e Protein, zTNF4 Protein, 2010006P15Rik Protein, Baffr Protein, Bcmd Protein, Bcmd-1 Protein, Bcmd1 Protein, Lvis22 Protein, zgc:172115 Protein, TNF superfamily member 13b Protein, TNF receptor superfamily member 13C Protein, tumor necrosis factor (ligand) superfamily, member 13b Protein, tumor necrosis factor receptor superfamily, member 13c Protein, tumor necrosis factor superfamily member 13b Protein, TNFSF13B Protein, TNFRSF13C Protein, Tnfsf13b Protein, Tnfrsf13c Protein, tnfsf13b Protein

Hintergrund: Synonyms: BLyS, THANK, TALL, zTNF4, TNFS20
BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.

Molekulargewicht: 18 - 20 kDa

Gen-ID: 10673, 603969

UniProt: Q9Y275

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response