LIN28A Protein (TAT tag)

Produktnummer ABIN2468762

Produktdetails

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses LIN28A Protein ist gelabelt mit TAT tag.

Sequenz: GPSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GP

Produktmerkmale: Biological activity was measured by its ability to induce fluorescence in Lin28 reporter cells (293 cells transfected with fluorescent protein genes under Lin28 control). Optimum activity was achieved at 20 μg/mL after incubation for 72 hr.

Reinheit: < 90 % by SDS-PAGE gel and HPLC analyses.

Endotoxin-Niveau: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Handhabung: As with any protein, exposing Lin28-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Lagerung: -20 °C

Informationen zur Lagerung: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Lin28-TAT stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Haltbarkeit: 24 months

Antigendetails

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Andere Bezeichnung: Lin28 (LIN28A Produkte)

Synonyme: CSDD1 Protein, LIN-28 Protein, LIN28 Protein, ZCCHC1 Protein, lin-28A Protein, AL024421 Protein, ENSMUSG00000070700 Protein, Gm10299 Protein, Lin-28 Protein, Lin28 Protein, Tex17 Protein, csdd1 Protein, lin-28 Protein, lin28 Protein, zcchc1 Protein, sb:cb650 Protein, zgc:55584 Protein, RGD1566408 Protein, lin-28 homolog A Protein, lin-28 homolog A (C. elegans) Protein, lin-28 homolog A S homeolog Protein, LIN28A Protein, Lin28a Protein, lin28a.S Protein, lin28a Protein

Hintergrund: Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28-TAT is a 24.4 kDa protein containing 222 amino acid residues, including 13- residue C-terminal TAT peptide.

Gen-ID: 79727

NCBI Accession: NP_078950

OMIM: 13375938

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance