PLAU Protein (AA 21-431) (His tag)

Produktnummer ABIN2181654

Produktdetails

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-431

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PLAU Protein ist gelabelt mit His tag.

Sequenz: AA 21-431

Produktmerkmale: Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 45 kDa. The predicted N-terminus is Ser21, Ile179 & Lys156. The reducing (R) protein migrates as four bands corresponding to the short α chain, long α chain, β chain and unprocessed full-length chain with the molecular mass of 20 kDa, 23 kDa, 32 kDa and 50 kDa respectively in SDS-PAGE due to glycosylation and cleavage.

Reinheit: >92 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Sodium Acetate, 100 mM NaCl, pH 5.0

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Referenzen

Ambrosetti, Paoletti, Bosco, Parisse, Scaini, Tagliabue, de Marco, Casalis: "Quantification of Circulating Cancer Biomarkers via Sensitive Topographic Measurements on Single Binder Nanoarrays." in: ACS omega, Vol. 2, Issue 6, pp. 2618-2629, (2017) (PubMed).

Antigendetails

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Andere Bezeichnung: PLAU (PLAU Produkte)

Synonyme: PLAU Protein, u-PA Protein, ATF Protein, BDPLT5 Protein, QPD Protein, UPA Protein, URK Protein, uPA Protein, UPAM Protein, plasminogen activator, urokinase Protein, urokinase-type plasminogen activator Protein, PLAU Protein, CpipJ_CPIJ002131 Protein, CpipJ_CPIJ006543 Protein, CpipJ_CPIJ013396 Protein, CpipJ_CPIJ013623 Protein, Plau Protein

Hintergrund: Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys - Pro - Gly - Arg560 - Val561 - Val - Gly - Gly - Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).

Molekulargewicht: 47.2 kDa

NCBI Accession: NP_002649

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie, Smooth Muscle Cell Migration