Sonic Hedgehog Protein (SHH) (AA 25-198)

Produktnummer ABIN2018370

Produktdetails

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-198

Spezies: Maus

Quelle: CHO Cells

Produktmerkmale: ED50 < 1 μg/mL, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of >1 x 10^3 units/mg.

Reinheit: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant Murine Sonic Hedgehog (SHH) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmSHH should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: Sonic Hedgehog (SHH)

Abstract: SHH Produkte

Synonyme: HHG1 Protein, HLP3 Protein, HPE3 Protein, MCOPCB5 Protein, SMMCI Protein, TPT Protein, TPTPS Protein, 9530036O11Rik Protein, Dsh Protein, Hhg1 Protein, Hx Protein, Hxl3 Protein, M100081 Protein, fc83d08 Protein, shh Protein, syu Protein, vhh-1 Protein, vhh1 Protein, wu:fc83d08 Protein, Xhh Protein, hedgehog Protein, xshh Protein, SHH Protein, twh Protein, twhh Protein, sonic hedgehog Protein, sonic hedgehog a Protein, sonic hedgehog L homeolog Protein, sonic hedgehog protein A Protein, sonic hedgehog b Protein, SHH Protein, Shh Protein, shha Protein, shh.L Protein, shh Protein, shhb Protein

Hintergrund: Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93 % identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Synonyms: SHH, HHG-1

Molekulargewicht: 20 kDa, observed by non-reducing SDS-PAGE.

UniProt: Q62226

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development