ATG4A Protein (AA 1-398) (His tag)

Produktnummer ABIN1692089

Produktdetails

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-398

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ATG4A Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Autophagy Related 4 Homolog A/ATG4A (N-6His)

Sequenz: MGSSHHHHHH SSGLVPRGSH MESVLSKYED QITIFTDYLE EYPDTDELVW ILGKQHLLKT EKSKLLSDIS ARLWFTYRRK FSPIGGTGPS SDAGWGCMLR CGQMMLAQAL ICRHLGRDWS WEKQKEQPKE YQRILQCFLD RKDCCYSIHQ MAQMGVGEGK SIGEWFGPNT VAQVLKKLAL FDEWNSLAVY VSMDNTVVIE DIKKMCRVLP LSADTAGDRP PDSLTASNQS KGTSAYCSAW KPLLLIVPLR LGINQINPVY VDAFKECFKM PQSLGALGGK PNNAYYFIGF LGDELIFLDP HTTQTFVDTE ENGTVNDQTF HCLQSPQRMN ILNLDPSVAL GFFCKEEKDF DNWCSLVQKE ILKENLRMFE LVQKHPSHWP PFVPPAKPEV TTTGAEFIDS TEQLEEFDLE EDFEILSV

Produktmerkmale:

Recombinant Human Autophagy Related 4 Homolog A/ATG4A (N-6His)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Andere Bezeichnung: Autophagy Related 4 Homolog A (ATG4A Produkte)

Synonyme: apg4a Protein, autl2 Protein, F6E13.27 Protein, APG4A Protein, zgc:111958 Protein, Aut2a Protein, AUTL2 Protein, AI627006 Protein, AV169859 Protein, Apg4a Protein, Atg4al Protein, Autl2 Protein, Atg4a Protein, autophagy related 4A cysteine peptidase L homeolog Protein, autophagy related 4A cysteine peptidase Protein, Peptidase family C54 protein Protein, ATG4 autophagy related 4 homolog A (S. cerevisiae) Protein, autophagy related 4A, cysteine peptidase Protein, atg4a.L Protein, ATG4A Protein, AT2G44140 Protein, atg4a Protein, Atg4a Protein

Hintergrund: Recombinant Human Cysteine Protease ATG4A/ATG4A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val398) of Human ATG4A fused with a 6His tag at the N-terminus.
Cysteine Protease ATG4A (ATG4A) is a cytoplasmic protein that belongs to the peptidase C54 family. ATG4A is widely expressed in many tissues at a low level, but the highest expression is observed in skeletal muscle and brain. ATG4A is a cysteine protease required for autophagy, it cleaves the C-terminal part of MAP1LC3, GABARAPL2 or GABARAP. ATG4A is inhibited by N-ethylmaleimide. It is suggested that ATG4A has a significant role in suppressing various cancers.

Molekulargewicht: 47.5 kDa

UniProt: Q8WYN0

Pathways: Autophagie