GRB2 Protein (AA 1-217) (His tag)

Produktnummer ABIN1096789

Produktdetails

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-217

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GRB2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Growth Factor Receptor-Bound Protein 2/GRB2/ASH (C-6His)

Sequenz: MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNVLEH HHHHH

Produktmerkmale: Recombinant Human Growth Factor Receptor-Bound Protein 2/GRB2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val217) of Human GRB2 fused with a 6His tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Andere Bezeichnung: GRB2 (GRB2 Produkte)

Synonyme: GRB2 Protein, grb2 Protein, AA408164 Protein, Ash Protein, ASH Protein, EGFRBP-GRB2 Protein, Grb3-3 Protein, MST084 Protein, MSTP084 Protein, NCKAP2 Protein, wu:fb18e12 Protein, wu:fb34e06 Protein, zgc:73077 Protein, growth factor receptor bound protein 2 Protein, growth factor receptor-bound protein 2 Protein, growth factor receptor-bound protein 2b Protein, GRB2 Protein, grb2 Protein, Grb2 Protein, grb2b Protein

Sub Type: Fusionprotein

Hintergrund: As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.
Alternative Names: Growth Factor Receptor-Bound Protein 2, Adapter Protein GRB2, Protein Ash, SH2/SH3 Adapter GRB2, GRB2, ASH

Molekulargewicht: 26.3 kDa

UniProt: P62993

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation