anti-Huhn p53 Antikörper für Immunocytochemistry

Recommended p53 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tumor Protein P53 (TP53) Antikörper
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
Epitop
AA 16-25, N-Term
169
124
78
74
72
71
70
59
58
54
51
46
45
43
35
34
34
25
24
24
22
22
21
21
17
15
15
15
15
15
15
15
13
13
13
12
11
10
9
9
8
8
8
8
7
7
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Huhn, Hund, Hamster, Human, Affe, Maus
2533
567
476
307
162
103
102
67
55
26
23
23
18
17
17
16
7
6
5
4
4
4
4
3
3
2
2
1
1
Wirt
Maus
1483
1166
7
5
1
1
1
1
Klonalität
Monoklonal
Konjugat
Dieser p53 Antikörper ist unkonjugiert
82
81
69
52
45
38
37
32
23
23
23
20
20
20
20
20
19
19
18
18
17
16
15
14
14
14
14
14
12
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
5
2
2
2
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
2088
962
864
782
681
662
420
316
266
179
110
90
75
41
32
17
17
15
13
10
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Hersteller
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Bilder

Produktnummer ABIN4342783
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN4342798 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342802 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342804 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342805 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342811 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342799 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342808 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342810 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342812 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342797 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342800 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342807 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342803 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342796 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN4342806 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB HRP Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN5946669 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 646 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0
1 ABIN5946668 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 549 Mouse IgG2a AA 16-25, N-Term Anmelden zum Anzeigen BP53-12 0

General

Antigen Tumor Protein P53 (TP53) Antikörper
Epitop AA 16-25, N-Term
(169), (124), (78), (74), (72), (71), (70), (59), (58), (54), (51), (46), (45), (43), (35), (34), (34), (25), (24), (24), (22), (22), (21), (21), (17), (15), (15), (15), (15), (15), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Huhn, Hund, Hamster, Human, Affe, Maus
(2533), (567), (476), (307), (162), (103), (102), (67), (55), (26), (23), (23), (18), (17), (17), (16), (7), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1)
Wirt Maus
(1483), (1166), (7), (5), (1), (1), (1), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p53 Antikörper ist unkonjugiert
(82), (81), (69), (52), (45), (38), (37), (32), (23), (23), (23), (20), (20), (20), (20), (20), (19), (19), (18), (18), (17), (16), (15), (14), (14), (14), (14), (14), (12), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (2), (2), (2)
Applikation Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(2088), (962), (864), (782), (681), (662), (420), (316), (266), (179), (110), (90), (75), (41), (32), (17), (17), (15), (13), (10), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p53 Antikörper

Target Details p53 Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN4342783) Bilder
Spezifität This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 Molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70 % but incidence varies from zero in carcinoid lung tumors to 97 % in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
Keine Kreuzreaktivität Maus, Ratte (Rattus)
Reinigung Protein G purified
Immunogen Full-length recombinant human p53 was used as immunogen for this antibody.
Klon BP53-12
Isotyp IgG2a
Plasmids, Primers & others

Target Details p53

Produktdetails anti-p53 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN4342783) Bilder zurück nach oben
Antigen
Andere Bezeichnung p53 (TP53 Antibody Abstract)
Hintergrund Gene Symbol: TP53
Molekulargewicht Theoretical MW: 53 kDa
Gen-ID 7157
UniProt P04637
Pathways p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie

Anwendungsinformationen

Produktdetails anti-p53 Antikörper Target Details p53 Handhabung Referenzen für anti-p53 Antikörper (ABIN4342783) Bilder zurück nach oben
Applikations-hinweise Western Blot 0.5-1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 0.5-1 μg/million cells in 0.1ml, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLHuman Chromosome Location: 17p13.1 Use in Immunoprecipitation has been reported in literature. Immunohistology (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Referenzen für anti-p53 Antikörper (ABIN4342783) Bilder zurück nach oben
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C.

Referenzen für anti-p53 Antikörper (ABIN4342783)

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Samaga, Rao, Chandrashekara Reddy, Kush, Diwakar: "Synthetic racemates of abyssinone I and II induces apoptosis through mitochondrial pathway in human cervix carcinoma cells." in: Bioorganic chemistry, Vol. 56, pp. 54-61, 2014 (Probematerial (Species): Human). Weitere Details: Western Blotting

Bilder

Produktdetails anti-p53 Antikörper Target Details p53 Anwendungsinformationen Handhabung Referenzen für anti-p53 Antikörper (ABIN4342783) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffi...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of MCF-7 cell line.
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Flow Cytometry: p53 Antibody (BP53-12) [NBP2-29453] - analysis of HT-29 cells using m...
Simple Western (SimWes) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows ...
Simple Western (SimWes) image for anti-Tumor Protein P53 (TP53) (AA 16-25), (N-Term) antibody (ABIN4342783) Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the c...