WISP1 CLIA Kit

Produktnummer ABIN5660035

Produktdetails WISP1 CLIA Kit

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of WNT1 Inducible Signaling Pathway Protein 1 (WISP1). No significant cross-reactivity or interference between WNT1 Inducible Signaling Pathway Protein 1 (WISP1) and analogues was observed.

Sensitivität: 1.21 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the WNT1 Inducible Signaling Pathway Protein 1 (WISP1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Andere Bezeichnung: WNT1 Inducible Signaling Pathway Protein 1 (WISP1 Produkte)

Synonyme: CCN4 CLIA Kit, WISP1c CLIA Kit, WISP1i CLIA Kit, WISP1tc CLIA Kit, WISP-1 CLIA Kit, WISP1 CLIA Kit, si:ch211-38f10.2 CLIA Kit, AW146261 CLIA Kit, Elm1 CLIA Kit, zgc:172276 CLIA Kit, WNT1 inducible signaling pathway protein 1 CLIA Kit, WNT1 inducible signaling pathway protein 1a CLIA Kit, WNT1-inducible-signaling pathway protein 1-like CLIA Kit, WNT1 inducible signaling pathway protein 1b CLIA Kit, WISP1 CLIA Kit, wisp1a CLIA Kit, Wisp1 CLIA Kit, LOC100627044 CLIA Kit, wisp1b CLIA Kit

Hintergrund:

Gene Name: WNT1 Inducible Signaling Pathway Protein 1

Gene Aliases: CCN4, WISP1c, WISP1i, WISP1tc, CCN family member 4, Wnt-1-induced secreted protein

Gen-ID: 22402

UniProt: O54775

Pathways: WNT Signalweg, Growth Factor Binding