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WISP1 ELISA Kit

WISP1 Reaktivität: Human AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Produktnummer ABIN1889373
  • Target Alle WISP1 ELISA Kits anzeigen
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Bindungsspezifität
    AA 23-367
    Reaktivität
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human WISP1/CCN4
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: T23-N367
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: T23-N367
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the CCN family.

    Tissue Specificity: Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.

    Plattentyp
    Pre-coated
    Protokoll
    human WISP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WISP1 has been precoated onto 96-well plates. Standards(NSO, T23-N367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WISP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human WISP1 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human WISP1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human WISP1 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 7.55, CV(%): 3.2
    • Sample 2: n=16, Mean(pg/ml): 1087, Standard deviation: 46.7, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 1826, Standard deviation: 89.5, CV(%): 4.9,
    • Sample 1: n=24, Mean(pg/ml): 352, Standard deviation: 16.2, CV(%): 4.6
    • Sample 2: n=24, Mean(pg/ml): 1240, Standard deviation: 64.5, CV(%): 5.2
    • Sample 3: n=24, Mean(pg/ml): 1910, Standard deviation: 128, CV(%): 6.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle WISP1 ELISA Kits anzeigen
    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
    Andere Bezeichnung
    WISP1 (WISP1 Produkte)
    Synonyme
    CCN4 ELISA Kit, WISP1c ELISA Kit, WISP1i ELISA Kit, WISP1tc ELISA Kit, WISP-1 ELISA Kit, WISP1 ELISA Kit, si:ch211-38f10.2 ELISA Kit, AW146261 ELISA Kit, Elm1 ELISA Kit, zgc:172276 ELISA Kit, WNT1 inducible signaling pathway protein 1 ELISA Kit, WNT1 inducible signaling pathway protein 1a ELISA Kit, WNT1-inducible-signaling pathway protein 1-like ELISA Kit, WNT1 inducible signaling pathway protein 1b ELISA Kit, WISP1 ELISA Kit, wisp1a ELISA Kit, Wisp1 ELISA Kit, LOC100627044 ELISA Kit, wisp1b ELISA Kit
    Hintergrund

    Protein Function: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. .

    Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.

    Synonyms: WNT1-inducible-signaling pathway protein 1,WISP-1,CCN family member 4,Wnt-1-induced secreted protein,WISP1,CCN4,

    Full Gene Name: WNT1-inducible-signaling pathway protein 1

    Cellular Localisation: Secreted.
    Gen-ID
    8840
    UniProt
    O95388
    Pathways
    WNT Signalweg, Growth Factor Binding
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