TNFRSF10B ELISA Kit

Produktnummer ABIN5659663

Produktdetails TNFRSF10B ELISA Kit

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) and analogues was observed.

Sensitivität: 0.062 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Andere Bezeichnung: Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B Produkte)

Synonyme: TNFRSF10B ELISA Kit, dr5 ELISA Kit, cd262 ELISA Kit, killer ELISA Kit, trick2 ELISA Kit, trickb ELISA Kit, xDR-M1 ELISA Kit, xDR-M2 ELISA Kit, ztnfr9 ELISA Kit, trailr2 ELISA Kit, trick2a ELISA Kit, trick2b ELISA Kit, CD262 ELISA Kit, DR5 ELISA Kit, KILLER ELISA Kit, KILLER/DR5 ELISA Kit, TRAIL-R2 ELISA Kit, TRAILR2 ELISA Kit, TRICK2 ELISA Kit, TRICK2A ELISA Kit, TRICK2B ELISA Kit, TRICKB ELISA Kit, ZTNFR9 ELISA Kit, Ly98 ELISA Kit, MK ELISA Kit, tumor necrosis factor receptor superfamily, member 10b ELISA Kit, tumor necrosis factor receptor superfamily member 10b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b L homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b ELISA Kit, TNF receptor superfamily member 10b ELISA Kit, Tnfrsf10b ELISA Kit, TNFRSF10B ELISA Kit, tnfrsf10b.S ELISA Kit, tnfrsf10b.L ELISA Kit, tnfrsf10b ELISA Kit

Hintergrund:

Gene Name: Tumor Necrosis Factor Receptor Superfamily, Member 10B

Gene Aliases: CD262, DR5, KILLER, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2

Pathways: p53 Signalweg, Apoptose, Positive Regulation of Endopeptidase Activity