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TNFRSF10B ELISA Kit
TNFRSF10B
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730110
Produktdetails TNFRSF10B ELISA Kit
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Target
Alle TNFRSF10B ELISA Kits anzeigen
TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
Reaktivität
Alle Reaktivitäten für TNFRSF10B ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse TRAIL R2/TNFRSF10B ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse TRAIL R2.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TNFRSF10B
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Target
Alle TNFRSF10B ELISA Kits anzeigen
TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
Andere Bezeichnung
TRAIL R2 (TNFRSF10B (TNFRSF10B Produkte )
Synonyme
TNFRSF10B ELISA Kit, dr5 ELISA Kit, cd262 ELISA Kit, killer ELISA Kit, trick2 ELISA Kit, trickb ELISA Kit, xDR-M1 ELISA Kit, xDR-M2 ELISA Kit, ztnfr9 ELISA Kit, trailr2 ELISA Kit, trick2a ELISA Kit, trick2b ELISA Kit, CD262 ELISA Kit, DR5 ELISA Kit, KILLER ELISA Kit, KILLER/DR5 ELISA Kit, TRAIL-R2 ELISA Kit, TRAILR2 ELISA Kit, TRICK2 ELISA Kit, TRICK2A ELISA Kit, TRICK2B ELISA Kit, TRICKB ELISA Kit, ZTNFR9 ELISA Kit, Ly98 ELISA Kit, MK ELISA Kit, tumor necrosis factor receptor superfamily, member 10b ELISA Kit, tumor necrosis factor receptor superfamily member 10b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b L homeolog ELISA Kit, tumor necrosis factor receptor superfamily member 10b ELISA Kit, TNF receptor superfamily member 10b ELISA Kit, Tnfrsf10b ELISA Kit, TNFRSF10B ELISA Kit, tnfrsf10b.S ELISA Kit, tnfrsf10b.L ELISA Kit, tnfrsf10b ELISA Kit
UniProt
Q9QZM4
Pathways
p53 Signalweg , Apoptose , Positive Regulation of Endopeptidase Activity
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