TIGIT ELISA Kit

Produktnummer ABIN5659080

Produktdetails TIGIT ELISA Kit

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). No significant cross-reactivity or interference between T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) and analogues was observed.

Sensitivität: 0.133 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Andere Bezeichnung: T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT Produkte)

Synonyme: VSIG9 ELISA Kit, VSTM3 ELISA Kit, WUCAM ELISA Kit, ENSMUSG00000071552 ELISA Kit, Vstm3 ELISA Kit, RGD1563191 ELISA Kit, T-cell immunoreceptor with Ig and ITIM domains ELISA Kit, T cell immunoreceptor with Ig and ITIM domains ELISA Kit, TIGIT ELISA Kit, Tigit ELISA Kit

Hintergrund:

Gene Name: T-Cell Immunoreceptor With Ig And ITIM Domains Protein

Gene Aliases: VSIG9, VSTM3, V-Set And Immunoglobulin Domain Containing 9, V-Set And Transmembrane Domain Containing 3

Gen-ID: 201633

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints