AGT CLIA Kit

Produktnummer ABIN5651811

Produktdetails AGT CLIA Kit

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT). No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.

Sensitivität: 0.03 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensinogen (AGT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensinogen (AGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensinogen (AGT) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Andere Bezeichnung: Angiotensinogen (AGT Produkte)

Synonyme: ANHU CLIA Kit, SERPINA8 CLIA Kit, AI265500 CLIA Kit, AngI CLIA Kit, AngII CLIA Kit, Aogen CLIA Kit, Serpina8 CLIA Kit, ANRT CLIA Kit, Ang CLIA Kit, PAT CLIA Kit, wu:fb62f06 CLIA Kit, wu:fj87b02 CLIA Kit, zgc:111892 CLIA Kit, AGT CLIA Kit, angt CLIA Kit, ANGT CLIA Kit, angiotensinogen CLIA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) CLIA Kit, AGT CLIA Kit, Agt CLIA Kit, agt CLIA Kit

Hintergrund:

Gene Name: Angiotensinogen

Gene Aliases: ANHU, SERPINA8, Angiotensin, Renin Substrate, Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

UniProt: P11859

Pathways: JAK-STAT Signalweg, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity