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AGT ELISA Kit
AGT
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4885084
Produktdetails AGT ELISA Kit
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Target
Alle AGT ELISA Kits anzeigen
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Reaktivität
Alle Reaktivitäten für AGT ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Angiotensinogen (SERPINA8) SpeedELISA Kit.
Marke
SpeedELISA
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Produktmerkmale
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Testdauer
3 h
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Haltbarkeit
6 months
Details zu AGT
(ausblenden)
Target
Alle AGT ELISA Kits anzeigen
AGT
(Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))
Andere Bezeichnung
Angiotensinogen (AGT Produkte )
Synonyme
ANHU ELISA Kit, SERPINA8 ELISA Kit, AI265500 ELISA Kit, AngI ELISA Kit, AngII ELISA Kit, Aogen ELISA Kit, Serpina8 ELISA Kit, ANRT ELISA Kit, Ang ELISA Kit, PAT ELISA Kit, wu:fb62f06 ELISA Kit, wu:fj87b02 ELISA Kit, zgc:111892 ELISA Kit, AGT ELISA Kit, angt ELISA Kit, ANGT ELISA Kit, angiotensinogen ELISA Kit, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) ELISA Kit, AGT ELISA Kit, Agt ELISA Kit, agt ELISA Kit
Gen-ID
183
UniProt
P01019
Pathways
JAK-STAT Signalweg , ACE Inhibitor Pathway , EGFR Signaling Pathway , Peptide Hormone Metabolism , Regulation of Systemic Arterial Blood Pressure by Hormones , Regulation of Lipid Metabolism by PPARalpha , Protein targeting to Nucleus , Feeding Behaviour , Monocarboxylic Acid Catabolic Process , Dicarboxylic Acid Transport , Positive Regulation of Response to DNA Damage Stimulus , Regulation of long-term Neuronal Synaptic Plasticity
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