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TRKA ELISA Kit

NTRK1 Reaktivität: Human Colorimetric Sandwich ELISA 2-500 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4884901
  • Target Alle TRKA (NTRK1) ELISA Kits anzeigen
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    Reaktivität
    • 9
    • 8
    • 5
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    2-500 ng/mL
    Untere Nachweisgrenze
    2 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human TrkA ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects human TrkA. Other species not determined.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle TRKA (NTRK1) ELISA Kits anzeigen
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    Andere Bezeichnung
    TrkA (NTRK1 Produkte)
    Synonyme
    MTC ELISA Kit, TRK ELISA Kit, TRK1 ELISA Kit, TRKA ELISA Kit, Trk-A ELISA Kit, p140-TrkA ELISA Kit, CDHF12 ELISA Kit, CDHR16 ELISA Kit, HSCR1 ELISA Kit, MEN2A ELISA Kit, MEN2B ELISA Kit, MTC1 ELISA Kit, PTC ELISA Kit, RET-ELE1 ELISA Kit, RET51 ELISA Kit, Trk ELISA Kit, C80751 ELISA Kit, Tkr ELISA Kit, TrkA ELISA Kit, trk ELISA Kit, trka ELISA Kit, CTRKA ELISA Kit, trkA ELISA Kit, neurotrophic receptor tyrosine kinase 1 ELISA Kit, ret proto-oncogene ELISA Kit, neurotrophic tyrosine kinase, receptor, type 1 ELISA Kit, NTRK1 ELISA Kit, RET ELISA Kit, Ntrk1 ELISA Kit, ntrk1 ELISA Kit
    Gen-ID
    4914
    UniProt
    P04629
    Pathways
    RTK Signalweg, Neurotrophin Signalübertragung, cAMP Metabolic Process
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