MEF2C ELISA Kit

Produktnummer ABIN4883753

Produktdetails MEF2C ELISA Kit

Target: MEF2C (Myocyte Enhancer Factor 2C (MEF2C))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human MEF2C ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu MEF2C

Target: MEF2C (Myocyte Enhancer Factor 2C (MEF2C))

Andere Bezeichnung: MEF2C (MEF2C Produkte)

Synonyme: MEF2C ELISA Kit, XMEF2C ELISA Kit, si:ch211-202e12.2 ELISA Kit, C5DELq14.3 ELISA Kit, DEL5q14.3 ELISA Kit, 5430401D19Rik ELISA Kit, 9930028G15Rik ELISA Kit, AV011172 ELISA Kit, Mef2 ELISA Kit, RGD1563119 ELISA Kit, id:ibd5026 ELISA Kit, mef2c ELISA Kit, wu:fc05b06 ELISA Kit, zgc:64184 ELISA Kit, zgc:85726 ELISA Kit, myocyte enhancer factor 2C ELISA Kit, MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C) ELISA Kit, myocyte enhancer factor 2cb ELISA Kit, MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C) L homeolog ELISA Kit, myocyte enhancer factor 2ca ELISA Kit, MEF2C ELISA Kit, mef2c ELISA Kit, mef2cb ELISA Kit, mef2c.L ELISA Kit, Mef2c ELISA Kit, mef2ca ELISA Kit

Gen-ID: 4208

UniProt: Q06413

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, BCR Signaling