IL12A ELISA Kit (Interleukin 12 alpha)

Details for Product IL12A ELISA Kit No. ABIN411310, Anbieter: Anmelden zum Anzeigen
Antigen
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • Il12a
  • IL12A
Epitop
AA 23-335, AA 23-215
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
16
12
11
7
6
6
4
4
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
15.6-1000 pg/mL
Untere Nachweisgrenze
15.6 pg/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-12(p70)
Marke PicoKine™
Proben Cell Culture Supernatant, Serum
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)
Spezifität Expression system for standard: sf21
Immunogen sequence: (M23-S335)+(R23-A215)
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <1.5pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Plasmids, Primers & others Plasmide, Primers & weitere IL12A products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung IL12A (IL12A ELISA Kit Abstract)
Hintergrund

Background: Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

Synonyms: Interleukin-12 subunit alpha ,Il12a ,

Full Gene Name: interleukin 12A

Gen-ID 16159
UniProt F8WI71
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plattentyp Pre-coated
Protokoll mouse IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(P70) amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(P70) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-12(P70) standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 97, Standard deviation: 6.5, CV(%): 6.7
  • Sample 2: n=16, Mean(pg/ml): 367, Standard deviation: 25.7, CV(%): 7
  • Sample 3: n=16, Mean(pg/ml): 633, Standard deviation: 34.2, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 123, Standard deviation: 9.23, CV(%): 7.5
  • Sample 2: n=24, Mean(pg/ml): 408, Standard deviation: 33.05, CV(%): 8.1
  • Sample 3: n=24, Mean(pg/ml): 657, Standard deviation: 38.8, CV(%): 5.9
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for Interleukin 12 alpha (IL12A) ELISA Kit (ABIN411310) Mouse IL-12(p70) PicoKine ELISA Kit standard curve
Produkt verwendet in: Liu, Xu, Chang, Qin, Yin, Yang: "4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-κB signaling in a ROS independent manner." in: Oncotarget, Vol. 7, Issue 27, pp. 40978-40991, 2018 (PubMed).

Lin, Chen, Xia, Yang: "MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3." in: Oncotarget, Vol. 7, Issue 31, pp. 48978-48994, 2018 (PubMed).

Wang, Liu, Wang, Yang, Li, Ding, Sun, Bai, Li: "Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via Notch Signaling and Strengthens Dendritic Cell Mediated T Lymphocyte Cytotoxicity on Colon Cancer Cell CT26-WT." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2305683, 2018 (PubMed).

Lin, Xia, Tu, Zhang, Zeng, Yang: "H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375." in: Frontiers in microbiology, Vol. 8, pp. 287, 2017 (PubMed).

Li, Fan, Xia, Xiao, Wang, Sun, Zhang, He: "The immunosuppressive characteristics of FB1 by inhibition of maturation and function of BMDCs." in: International immunopharmacology, Vol. 47, pp. 206-211, 2017 (PubMed).

Aziz, Cui, Das, Brown, Ardell, Febbraio, Pluskota, Han, Wu, Ballantyne, Smith, Cathcart, Yakubenko: "The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 12, pp. 4855-4867, 2017 (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, 2016 (PubMed).

Wang, Ji, Zhang, Fan, Zhang, Shi, Zhou, Chen, Wang, Wang: "Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells." in: Oncotarget, Vol. 6, Issue 42, pp. 44688-702, 2016 (PubMed).

Wen, Xiong, He, Zhang, Du, Liu, Wang, Zhou, Ma: "Fusion cytokine IL-2-GMCSF enhances anticancer immune responses through promoting cell-cell interactions." in: Journal of translational medicine, Vol. 14, pp. 41, 2016 (PubMed).

Huai, Dong, Zhu, Li, Cao, Gao, Yang, Wang, Mao: "Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection." in: Advanced healthcare materials, Vol. 5, Issue 7, pp. 786-94, 2016 (PubMed).

Li, Li, Liu, Zhuang, Yang, Zhang: "Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice." in: Oncology letters, Vol. 7, Issue 2, pp. 576-582, 2014 (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014 (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014 (PubMed).

Li, Li, Zuo, Zhou, Hao, Dang, Jiang, He, Deng: "Up-regulated expression of Tim-3/Gal-9 at maternal-fetal interface in pregnant woman with recurrent spontaneous abortion." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 4, pp. 586-90, 2014 (PubMed).

Ma, He, Han, Yang, Ji: "A clinical study of HBsAg-activated dendritic cells and cytokine-induced killer cells during the treatment for chronic hepatitis B." in: Scandinavian journal of immunology, Vol. 78, Issue 4, pp. 387-93, 2013 (PubMed).

Huang, Yang, Jiang, Shao, Sun, Chen, Dong, Zhang: "Anti-tumor immune responses of tumor-associated macrophages via toll-like receptor 4 triggered by cationic polymers." in: Biomaterials, Vol. 34, Issue 3, pp. 746-55, 2012 (PubMed).

Huang, Zhang, Jiang, Zhang, Chen, Dong, Zhang: "Targeted delivery of oligonucleotides into tumor-associated macrophages for cancer immunotherapy." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 158, Issue 2, pp. 286-92, 2012 (PubMed).

Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012 (PubMed).

Li, Li, Li, Deng, Tian, Jiang, Wang, Wang: "A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection." in: Biological & pharmaceutical bulletin, Vol. 35, Issue 10, pp. 1752-60, 2012 (PubMed).

Allgemeine Veröffentlichungen Shi, Chen, Wang, Zhu, Xu, Wang, Xiao, Shi, Zhang, Xu: "Short amyloid-beta immunogens show strong immunogenicity and avoid stimulating pro-inflammatory pathways in bone marrow-derived dendritic cells from C57BL/6J mice in vitro." in: Peptides, Vol. 32, Issue 8, pp. 1617-25, 2011 (PubMed).

Haben Sie etwas anderes gesucht?