IL-24 ELISA Kit

Produktnummer ABIN2703222

Produktdetails IL-24 ELISA Kit

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.41-100 ng/mL

Untere Nachweisgrenze: 0.41 ng/mL

Applikation: ELISA

Verwendungszweck: Human IL-24 ELISA Kit for Cell Culture Supernatants, EDTA and/or Citrate treated plasma, and Serum samples. Heparin is not recommended.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Human Interleukin-24.

Sensitivität: 0.41 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu IL-24

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Andere Bezeichnung: IL-24 (IL24 Produkte)

Synonyme: C49A ELISA Kit, FISP ELISA Kit, IL10B ELISA Kit, MDA7 ELISA Kit, MOB5 ELISA Kit, ST16 ELISA Kit, Mda7 ELISA Kit, St16 ELISA Kit, Mda-7 ELISA Kit, IL24 ELISA Kit, interleukin 24 ELISA Kit, IL24 ELISA Kit, Il24 ELISA Kit

Hintergrund: Gene Names: IL24 MDA7 ST16
Protein names: Interleukin-24 (IL-24) (Melanoma differentiation-associated gene 7 protein) (MDA-7) (Suppression of tumorigenicity 16 protein)

Gen-ID: 11009

UniProt: Q13007

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagie