APOA1 ELISA Kit

Produktnummer ABIN2702827

Produktdetails APOA1 ELISA Kit

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Human, Rhesusaffen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.08-20 ng/mL

Untere Nachweisgrenze: 0.08 ng/mL

Applikation: ELISA

Verwendungszweck: Human ApoA ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.

Sensitivität: 0.08 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples1,000,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

For example, add 1 μL of serum or plasma into a tube with 999 μL 1X Assay Diluent to prepare a 1,000-fold diluted sample. Mix thoroughly and then pipette 1 μL of prepared 1,000-fold diluted sample into a tube with 999 μL 1X Assay Diluent to prepare a final 1,000,000-fold diluted sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: APOA1 (APOA1 Produkte)

Synonyme: Alp-1 ELISA Kit, Apoa-1 ELISA Kit, Brp-14 ELISA Kit, Ltw-1 ELISA Kit, Lvtw-1 ELISA Kit, Sep-1 ELISA Kit, Sep-2 ELISA Kit, Sep2 ELISA Kit, apoA-I ELISA Kit, apoa ELISA Kit, apoa1 ELISA Kit, cb49 ELISA Kit, wu:fb33f01 ELISA Kit, zgc:103718 ELISA Kit, MGC64335 ELISA Kit, MGC89745 ELISA Kit, Apo-AI ELISA Kit, ApoA-I ELISA Kit, APOA1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, ApoAI ELISA Kit, apolipoprotein A1 ELISA Kit, apolipoprotein A-I ELISA Kit, apolipoprotein A-Ia ELISA Kit, apolipoprotein A-I S homeolog ELISA Kit, apolipoprotein A4 ELISA Kit, APOA1 ELISA Kit, Apoa1 ELISA Kit, apoa1a ELISA Kit, apoa1.S ELISA Kit, apoa1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, apoa4 ELISA Kit

Hintergrund: Gene Names: APOA1
Protein names: Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I), Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]

Gen-ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism