APOA1 ELISA Kit

Produktnummer ABIN6975327

Produktdetails APOA1 ELISA Kit

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 ng/mL - 2000 ng/mL

Untere Nachweisgrenze: 31.25 ng/mL

Applikation: ELISA

Verwendungszweck: For the quantitative determination of mouse apolipoprotein A1 (Apo-A1) concentrations in serum, plasma, cell culture supernates, tissue homogenates, urine.

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of mouse Apo-A1. No significant cross-reactivity or interference between mouse Apo-A1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse Apo-A1 and all the analogues, therefore, cross reaction may still exist.

Sensitivität: 7.81 ng/mL

Bestandteile:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Aufbereitung der Reagenzien:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 2000 ng/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (2000 ng/mL). Sample Diluent serves as the zero standard (0 ng/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Note:

Recommend to dilute the serum or plasma samples with Sample Diluent (1:2000) before test. The suggested 2000-fold dilution can be achieved by adding 2 μL sample to 98 μL of Sample Diluent. Complete the 2000-fold dilution by adding 6 μL of this solution to 234 μL of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments.

Testpräzision: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Haltbarkeit: 6 months

Referenzen für APOA1 ELISA Kit (ABIN6975327)

Du, Jiang, Zeng, Zhang, Pan, Zhou, Xie, Kan, Song, Sun, Zhao: "Air pollution is associated with the development of atherosclerosis via the cooperation of CD36 and NLRP3 inflammasome in ApoE-/- mice." in: Toxicology letters, Vol. 290, pp. 123-132, (2018) (PubMed).

Medina-Echeverz, Fioravanti, Díaz-Valdés, Frank, Aranda, Gomar, Ardaiz, Dotor, Umansky, Prieto, Berraondo: "Harnessing high density lipoproteins to block transforming growth factor beta and to inhibit the growth of liver tumor metastases." in: PLoS ONE, Vol. 9, Issue 5, pp. e96799, (2015) (PubMed).

Hu, Hu, Zhao, Li, Ma, Wu, Lu, Qiu, Sha, Wang, Gao, Zheng, Wang: "An agomir of miR-144-3p accelerates plaque formation through impairing reverse cholesterol transport and promoting pro-inflammatory cytokine production." in: PLoS ONE, Vol. 9, Issue 4, pp. e94997, (2015) (PubMed).

Hu, Yang, Ma, Chen, Hu, Zhao, Li, Qiu, Lu, Wang, Gao, Sha, Zheng, Wang: "A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis." in: Journal of lipid research, Vol. 55, Issue 4, pp. 681-97, (2014) (PubMed).

Ochoa, Fioravanti, Duitman, Medina-Echeverz, Palazon, Arina, Dubrot, Alfaro, Morales-Kastresana, Murillo, Hervas-Stubbs, Prieto, Berraondo, Melero: "Liver gene transfer of interkeukin-15 constructs that become part of circulating high density lipoproteins for immunotherapy." in: PLoS ONE, Vol. 7, Issue 12, pp. e52370, (2013) (PubMed).

Hu, Ma, Huang, Mao, Yang, Zhao, Li, Qiu, Yang, Zheng, Wang: "Dihydrocapsaicin Attenuates Plaque Formation through a PPAR?/LXR? Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet." in: PLoS ONE, Vol. 8, Issue 6, pp. e66876, (2013) (PubMed).

Wang, Xia, Gao, Li, Zhang, Jin, Ling: "Cyanidin-3-O-β-glucoside upregulates hepatic cholesterol 7α-hydroxylase expression and reduces hypercholesterolemia in mice." in: Molecular nutrition & food research, Vol. 56, Issue 4, pp. 610-21, (2012) (PubMed).

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: apolipoprotein A-I (APOA1 Produkte)

Synonyme: Alp-1 ELISA Kit, Apoa-1 ELISA Kit, Brp-14 ELISA Kit, Ltw-1 ELISA Kit, Lvtw-1 ELISA Kit, Sep-1 ELISA Kit, Sep-2 ELISA Kit, Sep2 ELISA Kit, apoA-I ELISA Kit, apoa ELISA Kit, apoa1 ELISA Kit, cb49 ELISA Kit, wu:fb33f01 ELISA Kit, zgc:103718 ELISA Kit, MGC64335 ELISA Kit, MGC89745 ELISA Kit, Apo-AI ELISA Kit, ApoA-I ELISA Kit, APOA1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, ApoAI ELISA Kit, apolipoprotein A1 ELISA Kit, apolipoprotein A-I ELISA Kit, apolipoprotein A-Ia ELISA Kit, apolipoprotein A-I S homeolog ELISA Kit, apolipoprotein A4 ELISA Kit, APOA1 ELISA Kit, Apoa1 ELISA Kit, apoa1a ELISA Kit, apoa1.S ELISA Kit, apoa1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, apoa4 ELISA Kit

Hintergrund:

Abbreviation: APOA1

Alias: MGC117399, OTTHUMP00000069346|OTTHUMP00000069347|OTTHUMP00000069348

UniProt: Q00623

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism